Solid-state NMR and membrane proteins.

 In
by Stanley J Opella
Abstract:
The native environment for a membrane protein is a phospholipid bilayer. Because the protein is immobilized on NMR timescales by the interactions within a bilayer membrane, solid-state NMR methods are essential to obtain high-resolution spectra. Approaches have been developed for both unoriented and oriented samples, however, they all rest on the foundation of the most fundamental aspects of solid-state NMR, and the chemical shift and homo- and hetero-nuclear dipole-dipole interactions. Solid-state NMR has advanced sufficiently to enable the structures of membrane proteins to be determined under near-native conditions in phospholipid bilayers.
Reference:
Solid-state NMR and membrane proteins. (Stanley J Opella), In Journal of magnetic resonance (San Diego, Calif. : 1997), volume 253, 2015.
Bibtex Entry:
@article{Opella2015,
abstract = {The native environment for a membrane protein is a phospholipid bilayer. Because the protein is immobilized on NMR timescales by the interactions within a bilayer membrane, solid-state NMR methods are essential to obtain high-resolution spectra. Approaches have been developed for both unoriented and oriented samples, however, they all rest on the foundation of the most fundamental aspects of solid-state NMR, and the chemical shift and homo- and hetero-nuclear dipole-dipole interactions. Solid-state NMR has advanced sufficiently to enable the structures of membrane proteins to be determined under near-native conditions in phospholipid bilayers.},
author = {Opella, Stanley J},
doi = {10.1016/j.jmr.2014.11.015},
issn = {1096-0856},
journal = {Journal of magnetic resonance (San Diego, Calif. : 1997)},
keywords = {Bilayers,Chemical shift anisotropy,Dipolar coupling,Magic angle spinning,Phospholipids,Structure determination},
month = {apr},
pages = {129--37},
pmid = {25681966},
title = {{Solid-state NMR and membrane proteins.}},
url = {http://linkinghub.elsevier.com/retrieve/pii/S109078071400336X http://www.ncbi.nlm.nih.gov/pubmed/25681966 http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC4372479},
volume = {253},
year = {2015}
}

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