Probe Performance

The following table summarizes the performance data for a 50mm double resonance probe operating at 750 MHz.

5 mm ID x 9 mm L coil for 750M magnet	1H	15N
Maximum tested power (input to the probe)	108 Watts	600 Watts
B1 field strength	88 kHz	72 kHz
Tuning Range	795M - 739M	72.5M - 85.4M
Probe Q with crystal sample	> 210	> 120
Probe Q with concentrated protein sample	~ 65	> 120
Tested VT capability	-50C to 65C

The following table summarizes general probe performance.

Probe Coil Geometry	Channel	Performance, Crystalline Sample: Nutation Frequency (kHz) Power Deposition (watts)	Performance, Biological Sample: Nutation Frequency (kHz) Power Deposition (watts)
750 Mhz Solenoid Coil	1H	85 – 90 kHz 100 – 125 watts	65 – 70 kHz 100 – 125 watts
	15N	70 – 75 kHz 600 – 700 watts	70 – 75 kHz 600 – 700 watts
750 Mhz Flat Coil	1H	50 – 55 Khz 75 – 100 watts	50 – 55 kHz 100 – 125 watts
	15N	50 – 55 kHz 700 – 800 watts	50 – 55 kHz 700 – 800 watts

Results

The PISEMA spectrum shown is from a uniform 15N labeled sample of PF1 bacteriophage aligned by the magnetic field of the NMR spectrometer. The 1H nutation frequency is 66 kHz compared to 87.7 kHz for 15N labeled N-acetyl-lucine single-crystal sample at the same power level. The nutation frequency remains the same for 15N channel.